How to get dna quantities from an agarose gel ?

Posted by Eddie Iannuccelli on Jan 15, 2008; 11:21am
URL: http://imagej.273.s1.nabble.com/How-to-get-dna-quantities-from-an-agarose-gel-tp3697585.html

Hello,
I am trying to find the easiest way to get dna quantities from agarose
gels bands, the gel provide one or more quantities control bands. The
ImageJ gel menu method return only areas  that are not corrected by
calibration, isn' it ?. Currently I first calibrate image using 3 or 4
control bands (which function best fit dna quantification ?) then I
manually select bands before measuring them  (or threshold  + particle
analyzer if bands intensity are not too heterogeneous) in order to get
the Integrated Intensity which represent the dna quantity.
Do you know a better method or perhaps  a plugin that do that better?

Regards

******************************************
Eddie Iannuccelli
Laboratoire de génétique cellulaire
INRA - Castanet Tolosan
Tel: [phone] / Fax: [phone]