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Re: Registering two stacks and before merging

Posted by Knecht, David on Jan 11, 2008; 7:09pm
URL: http://imagej.273.s1.nabble.com/Registering-two-stacks-and-before-merging-tp3697601p3697603.html

I am pretty sure it only does one at a time.  It would be really nice  
if it were updated to do the entire stack.  Dave

On Jan 11, 2008, at 1:27 PM, Tony Collins wrote:

> I think Gabriel's "Align RGB planes" plugin
> (http://www.dentistry.bham.ac.uk/landinig/software/software.html)  
> nearly
> does what you want, in that you can align the channels after merging.
> I'm not sure if it will align all slices at once or one at a time
> though.
> Maybe a small tweak?
> Best,
> Tony
>
>
> Tony J. Collins, Ph.D.
> McMaster Biophotonics Facility
> Dept. Biochemistry and Biomedical Sciences HSC 4H21A
> McMaster University, Hamilton, ON, L8N 3Z5
> (905) 525 9140 x28812(off.)/x26488(lab)
> [hidden email]     www.macbiophotonics.ca
>
>
>> -----Original Message-----
>> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
>> Christophe Leterrier
>> Sent: January 11, 2008 11:27 AM
>> To: [hidden email]
>> Subject: Registering two stacks and before merging
>>
>> Hi,
>>
>> I would like to merge two stacks (red and green) but one of them  
>> has a
>> constant drift, that is one is shifted off a few pixels compared to
> the
>> other. Do you know any function/macro/plugin that allos to
> interactively
>> superpose the two stacks (based on the two first images) to get the
> offset
>> visually, then actually merge the two stacks ? For the moment I use
> canvas
>> extension and cropping but I have to do a trial/error procedure,  
>> pixel
> by
>> pixel to estimate the offset...
>>
>> Thank you for your ideas,
>>
>> Christophe Leterrier
>>
>> Postdoc
>> INSERM UMR641 Neurobiology of ionic channels
>> IFR Jean Roche - Mediterranee University
>> Marseille, France

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)