Posted by
Piotr Wendykier on
Jan 07, 2008; 1:26pm
URL: http://imagej.273.s1.nabble.com/Parallel-Spectral-Deconvolution-plugin-update-tp3697631p3697633.html
Dear Christophe,
The short answer is: yes, it could be used for your Z-stacks,
and no it is not a totally different kind of deconvolution.
The long answer is harder to explain. There is just one definition of
deconvolution, but there are a lot of different methods that can be used
to actually do the deconvolution. Furthermore, some people use different
names for essentially the same methods. Why different methods? That's
hard to explain without using a lot of mathematics. The basic idea is that
the result produced by deconvolution algorithms depends on the image, the
noise in the image, and the PSF. One deconvolution method might work well
for one problem, but not so well for another problem. So it is good to
try different methods. This plugin uses fairly standard deconvolution
methods, with two advantages. First, we provide different deconvolution
methods that you can try with your data -- maybe one will work better
than another. Second, our methods have been implemented to be very
efficient on modern multi-core processor machines.
If you want to know more about these algorithms, I recommend an excellent
book: "Deblurring Images: Matrices, Spectra, and Filtering" by
Per Christian Hansen, James G. Nagy, and Dianne P. O'Leary, SIAM 2006.
Piotr
On Jan 7, 2008 4:36 AM, Christophe Leterrier
<
[hidden email]> wrote:
> I went to the plugin homepage, it seems very interesting but it didn't
> explained what is the use of such a decouvolution... Could someone explain
> what exactly is this plugin doing ? I'm interested in 3D deconvolution of
> widefield fluorescence Z-stacks, could this be used for this application or
> is it a totally different kind of deconvolution ?
>
>
> On Jan 7, 2008 12:10 AM, Piotr Wendykier <
[hidden email]> wrote:
>
> > Hello,
> >
> > I have updated the Parallel Spectral Deconvolution plugin.
> > The bug causing poor results in all 3D methods with reflexive
> > boundary conditions has been fixed. Moreover, the source code
> > has been significantly simplified and the plugin now uses Parallel Colt
> > 0.2.
> >
> > The plugin is available at:
> >
> >
http://piotr.wendykier.googlepages.com/deconvolution> >
> > Regards,
> >
> > Piotr Wendykier
> >
>