Re: Particle analysis: classifying with respect to size

Posted by Gabriel Landini on Dec 14, 2007; 8:26am
URL: http://imagej.273.s1.nabble.com/Particle-analysis-classifying-with-respect-to-size-tp3697736p3697739.html

On Friday 14 December 2007 06:07:43 Jacqui Ross wrote:
> I was wondering if anyone had written a macro or plugin for particle
> analysis which automatically uses mean individual particle size to
> determine and classify objects as being greater than one particle.

This may help:

http://www.dentistry.bham.ac.uk/landinig/software/classify/classify.html

> At the moment, my idea is to find out the mean (+ 2SD, etc.) using
> Analyze Particles and then go into Excel to classify them as one or more
> but if anyone has a better way of doing this, it would be great.

That would only work depending on 1. the variability of the particle size and
2. the degree of overlap between particles (some particles may not even have
a normal size distribution!).
I do not believe that one should use a canned solution to that problem. You
could estimate which deviation from the mean size corresponds to 2, 3
particles and so on. To do that you can label some images by hand and see at
the size distribution of the particles.

I hope it helps.

G.