Re: Particle analysis: classifying with respect to size

Posted by Jacqueline Ross on Dec 17, 2007; 5:38am
URL: http://imagej.273.s1.nabble.com/Particle-analysis-classifying-with-respect-to-size-tp3697736p3697740.html

Thanks very much for that Gabriel,

Your plugin looks like what I am looking for. I'll give it a run. I hope
you won't mind if I come back with additional questions!

Kind regards,

Jacqui


Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.health.auckland.ac.nz/biru/ 


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Gabriel Landini
Sent: Friday, 14 December 2007 9:26 p.m.
To: [hidden email]
Subject: Re: Particle analysis: classifying with respect to size

On Friday 14 December 2007 06:07:43 Jacqui Ross wrote:
> I was wondering if anyone had written a macro or plugin for particle
> analysis which automatically uses mean individual particle size to
> determine and classify objects as being greater than one particle.

This may help:

http://www.dentistry.bham.ac.uk/landinig/software/classify/classify.html

> At the moment, my idea is to find out the mean (+ 2SD, etc.) using
> Analyze Particles and then go into Excel to classify them as one or
> more but if anyone has a better way of doing this, it would be great.

That would only work depending on 1. the variability of the particle
size and 2. the degree of overlap between particles (some particles may
not even have a normal size distribution!).
I do not believe that one should use a canned solution to that problem.
You could estimate which deviation from the mean size corresponds to 2,
3 particles and so on. To do that you can label some images by hand and
see at the size distribution of the particles.

I hope it helps.

G.