Login  Register

Re: k-means Clustering

Posted by Gabriel Landini on Dec 10, 2007; 10:20am
URL: http://imagej.273.s1.nabble.com/k-means-Clustering-tp3697801p3697805.html

On Monday 10 December 2007 10:02:41 Richard Han wrote:
> I would like to measure the area of red/blue from RGB images of
> histological slides.

Histological stains behave as "subtractive" colours and it is very likely that
they will colocalise in some places.
If that is the case, colour thresholding does not work because the hue of the
co-localised stains is neither red nor blue (as it has contributions from
both) while the thresholding will be assigning a pixel to one of the 2
classes.

Have a look at Ruifrok's paper on colour deconvolution (I wrote a plugin that
does that):

Ruifrok AC, Johnston DA. Quantification of histochemical staining by color
deconvolution. Anal Quant Cytol Histol. 2001 Aug;23(4):291-9.

Regards,

G.