Measuring Volume

Posted by Aaron Johnson-9 on Dec 06, 2007; 3:47pm
URL: http://imagej.273.s1.nabble.com/Measuring-Volume-tp3697814.html

Hello, I'm interested in finding a way to do a volume measurement of an 8-
bit grayscale stack (usually between 15-20 images) coming from a confocal
microscope.  The structure I'm measuring (ACh receptor) is fairly
contiguous and could be characterized as blob-shaped.  My main questions
are:

1) What are your recommendations regarding thresholding?  I always want to
capture the max (255), and am more concerned about finding an automated
way of finding the minimum.  The "auto-threshold" doesn't work for me.

2) What do you recommend for the volume measurement?  I've tried voxel
counter with some success, but I'm wondering if there are other easy ways
to do this, if for no other reason than to confirm my measurements with
voxel counter.

Thanks!
Aaron