thick Golgi stained sections.
> Dear NeuronJ users,
>
> Just a short note to let you know that I released a new update of NeuronJ
> (version 1.3). The previous update (1.2) was released last July. Most
> changes compared to last year's update (1.1) are really minor (I do have
> lost of ideas for a major update but have no time to work on it currently...
> sorry), but there are a few important improvements:
>
> - Neurite centerline tracing in gray-scale (not 8-bit color) images showing
> clipped intensities (overexpression) is now much better (previously, the
> tracings were pushed away from the centerlines and towards the neurite edges
> in these cases, because pixels with RGB value 0xFFFFFF corresponded to
> background in the color images that I worked with, but this was a wrong
> assumption in gray-scale images of course).
>
> - I have added the possibility to export tracings as segmented line
> selections (polyline ROIs), which enables you to import them as ROIs into
> the images outside of NeuronJ and do all kinds of other measurements (such
> as intensity profile plotting).
>
> - The measurements dialog now also offers the possibility to do vertex
> measurements (coordinates/values) and to show either calibrated or
> noncalibrated results.
>
> As usual, the latest version can be downloaded from:
>
http://www.imagescience.org/meijering/software/neuronj/>
> Please note that it requires ImageJ 1.38x and Java 1.4 or higher.
>
> Best wishes,
>
> Erik
>
>
> On Wed, 27 Sep 2006 03:41:44 -0400, Erik Meijering wrote:
> >Dear NeuronJ users,
> >
> >In response to several posts regarding pixel calibration:
> >
> >With the previous version of NeuronJ (1.0), the pixel calibration (pixel
> >sizes and units) had to be set using the Parameters dialog of NeuronJ.
> >
> >With the latest version (1.1.0) of the program, these parameters can no
> >longer be specified using the Parameters dialog. Since these are
> >image-specific parameters, they can instead be set in the ImageJ > Image >
> >Properties dialog. The values of these parameters stored in older NeuronJ
> >data files are ignored.
> >
> >The latter version of NeuronJ also offers additional features. Please check
> >out the NeuronJ webpage for more details:
> >
http://www.imagescience.org/meijering/software/neuronj/> >
> >Best regards,
> >
> >Erik
>