Re: 4-colour merge?

Posted by John Alexander-7 on Nov 12, 2007; 5:37pm
URL: http://imagej.273.s1.nabble.com/4-colour-merge-tp3698028p3698037.html

I have found that a quick way to merge images of non-RGB colors is to
use the Past Control and set it to Add ...



John

Senger Fabrice wrote:

> Justin McGrath a écrit :
>> Joachim Walter wrote a nice plugin called Image5D.  It's found at
>> http://rsb.info.nih.gov/ij/plugins/image5d.html .  You can load your
>> four channels and pick a color for each.  I don't know much about
>> colocalization or quantifying fluorescence, but I would only use this
>> to make demonstration images.
>>
>> Justin
>>
>>
>>
>> On Nov 10, 2007 3:25 PM, Shane Harding <[hidden email]> wrote:
>>  
>>> Hi All,
>>> I'm a relatively new ImageJ user and would like to merge 4-colours
>>> into one composite image (CY3, CY5, Alexa488 and DAPI). Is this
>>> possible in ImageJ? How would I do this?
>>>
>>> Thank you,
>>> Shane
>>>
>>>    
> Hi there,
>
> to only make a demo image you could use aswell Photoshop or the freely
> available "the gimp" and work around with overlays.
>
> Sheers,
>
> Fabrice.
>

--
John K. Alexander, Ph.D.
Post-Doctoral Fellow
William Green Laboratory
University of Chicago
Dept. Neurobiology, Pharmacology, and Physiology
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