Re: 4-colour merge?

Posted by Michael Weber-4 on
URL: http://imagej.273.s1.nabble.com/4-colour-merge-tp3698028p3698038.html

Shane,

there is a plugin called "Colour Merge" which can merge every colour,
also violet plus yellow and so on.

As already mentioned, such an extensive overlay doesn't make sense for
quantitation. In my opinion one can use it if the structures are labeled
with one colour at once only, i.e. nuclei, membrane, endosomes etc.
separately - to get an overview of the situation in the cell/tissue.

cheers,
Michael


Justin McGrath wrote:

> Joachim Walter wrote a nice plugin called Image5D.  It's found at
> http://rsb.info.nih.gov/ij/plugins/image5d.html .  You can load your
> four channels and pick a color for each.  I don't know much about
> colocalization or quantifying fluorescence, but I would only use this
> to make demonstration images.
>
> Justin
>
>
>
> On Nov 10, 2007 3:25 PM, Shane Harding <[hidden email]> wrote:
>> Hi All,
>> I'm a relatively new ImageJ user and would like to merge 4-colours
>> into one composite image (CY3, CY5, Alexa488 and DAPI). Is this
>> possible in ImageJ? How would I do this?
>>
>> Thank you,
>> Shane