Re: 4-colour merge?

Posted by Shane Harding-2 on
URL: http://imagej.273.s1.nabble.com/4-colour-merge-tp3698028p3698039.html

Thank you all for your help.

I think the solution to my question is to use the DAPI image to create  
a boundary in which I can use the RGB labels. Is there an easy way to  
create an image with the DAPI regions outlined so that I may overlay  
my other 3 RGB coloured on this?

I agree that pairwise will also work, I'm just searching for  
different, but acceptable options.

Thanks Again,
Shane


Quoting Michael Weber <[hidden email]>:

> Shane,
>
> there is a plugin called "Colour Merge" which can merge every colour,
> also violet plus yellow and so on.
>
> As already mentioned, such an extensive overlay doesn't make sense for
> quantitation. In my opinion one can use it if the structures are
> labeled with one colour at once only, i.e. nuclei, membrane, endosomes
> etc. separately - to get an overview of the situation in the
> cell/tissue.
>
> cheers,
> Michael
>
>
> Justin McGrath wrote:
>> Joachim Walter wrote a nice plugin called Image5D.  It's found at
>> http://rsb.info.nih.gov/ij/plugins/image5d.html .  You can load your
>> four channels and pick a color for each.  I don't know much about
>> colocalization or quantifying fluorescence, but I would only use this
>> to make demonstration images.
>>
>> Justin
>>
>>
>>
>> On Nov 10, 2007 3:25 PM, Shane Harding <[hidden email]> wrote:
>>> Hi All,
>>> I'm a relatively new ImageJ user and would like to merge 4-colours
>>> into one composite image (CY3, CY5, Alexa488 and DAPI). Is this
>>> possible in ImageJ? How would I do this?
>>>
>>> Thank you,
>>> Shane