http://imagej.273.s1.nabble.com/Re-D-reconstruction-of-volume-tp3698096.html
Inverting would have been my first thought. If cytoplasm and membrane
and play with opacities.
> From: David Knecht-charter <
[hidden email]>
> Date: October 24, 2007 8:46:47 PM PDT (CA)
> Subject: 3D reconstruction of volume
>
>
> We are trying to make volume projections of some confocal z slices
> through a cell. There is cytoplasmic label and some concentrated
> membrane labeling. There are also large vacuoles and the nucleus
> where there is no label. Is there any way to get those black holes
> in the cytoplasm to show up as defined structures in the
> projections? They are obscured by the cytoplasmic label in a
> standard projection because they are of much lower intensity. If
> you try to project just hte lower intensity values, they are
> obscured by the fact that the extracellular background is equally
> black. Any ideas? Thanks- Dave
>
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>