Merging Stacks of different colors

Posted by Michael Jacobsen-3 on Oct 31, 2007; 3:02pm
URL: http://imagej.273.s1.nabble.com/Merging-Stacks-of-different-colors-tp3698097.html

Hello,

This is a first-time post to the ImageJ list.

I have been performing timelapse studies using the MetaMorph software
package. I am looking at two channels--GFP (FITC) and mCherry (Texas Red)--
and capturing images for 12 hours at 5-minute intervals.

The captured data are stored in monochrome format. I know how to open the
data in ImageJ as stacks, but how can I do the following?
-Pseudocolor the GFP stack green and mCherry stack red?
-Merge the green stack with the red stack?
-Save the final stack as one red-green merged stack?

Thank you very much,

Michael Jacobsen
Manager, Confocal Microscopy Core Facility
Department of Pharmacology and Experimental Neuroscience
University of Nebraska, Medical Center