Posted by
Koh Geok Liang, Esther on
Nov 01, 2007; 4:31am
URL: http://imagej.273.s1.nabble.com/Merging-Stacks-of-different-colors-tp3698097p3698098.html
Hello Michael,
To assign colour for each stack, click 'Image/Lookup Tables/' and choose the colour you want.
To merge the two stacks, you have to convert the stacks to RGB format:
click 'Image/Type/RGB Color'
Then merge the 2 RGB Color stacks by clicking 'Process/Image Calculator'
This brings up a pop-up window for you to choose the stacks you want to 'add'
You can save the merged stack as a tiff file.
cheers,
Esther Koh
Institute of Medical Biology
8A Biomedical Grove
#06-06 Immunos
Singapore 138648
-----Original Message-----
From: ImageJ Interest Group on behalf of Michael Jacobsen
Sent: Wed 10/31/2007 10:02 PM
To:
[hidden email]
Subject: Merging Stacks of different colors
Hello,
This is a first-time post to the ImageJ list.
I have been performing timelapse studies using the MetaMorph software
package. I am looking at two channels--GFP (FITC) and mCherry (Texas Red)--
and capturing images for 12 hours at 5-minute intervals.
The captured data are stored in monochrome format. I know how to open the
data in ImageJ as stacks, but how can I do the following?
-Pseudocolor the GFP stack green and mCherry stack red?
-Merge the green stack with the red stack?
-Save the final stack as one red-green merged stack?
Thank you very much,
Michael Jacobsen
Manager, Confocal Microscopy Core Facility
Department of Pharmacology and Experimental Neuroscience
University of Nebraska, Medical Center