3D reconstruction of volume

Posted by Knecht, David on Oct 25, 2007; 4:46am
URL: http://imagej.273.s1.nabble.com/3D-reconstruction-of-volume-tp3698102.html

We are trying to make volume projections of some confocal z slices  
through a cell.  There is cytoplasmic label and some concentrated  
membrane labeling.  There are also large vacuoles and the nucleus  
where there is no label.  Is there any way to get those black holes  
in the cytoplasm to show up as defined structures in the  
projections?  They are obscured by the cytoplasmic label in a  
standard projection because they are of much lower intensity.  If you  
try to project just hte lower intensity values, they are obscured by  
the fact that the extracellular background is equally black.  Any  
ideas?  Thanks- Dave


Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)