http://imagej.273.s1.nabble.com/3D-reconstruction-of-volume-tp3698102p3698110.html
Here's another idea. What would happen if you invert the images?
and vice versa. You could then reconstruct that volume, in which the
images. I have done something like this with brightfield images of
H&E stained tissue. You can see an example on our department's home
> David,
> You could draw a ROI ( wedge) from the interior outward and clear the
> ROI (option to do so for entire stack). This way it looks like a cut pie
> upon 3D projection. You could play with opacity and surface/interior
> weights to emphasize the inner features, though I've not had great
> success this way. You should also try the Volume Viewer/3D Slicer and/or
> VolumeJ plugins that can perform this type of rendering option (ROI not
> required). Animating a series of XZ or YZ reslice projections would show
> what you want to show, too.
>
>
> David Knecht-charter wrote:
> > We are trying to make volume projections of some confocal z slices
> > through a cell. There is cytoplasmic label and some concentrated
> > membrane labeling. There are also large vacuoles and the nucleus
> > where there is no label. Is there any way to get those black holes in
> > the cytoplasm to show up as defined structures in the projections?
> > They are obscured by the cytoplasmic label in a standard projection
> > because they are of much lower intensity. If you try to project just
> > hte lower intensity values, they are obscured by the fact that the
> > extracellular background is equally black. Any ideas? Thanks- Dave
> >
> >
> > Dr. David Knecht
> > Department of Molecular and Cell Biology
> > Co-head Flow Cytometry and Confocal Microscopy Facility
> > U-3125
> > 91 N. Eagleville Rd.
> > University of Connecticut
> > Storrs, CT 06269
> > 860-486-2200
> > 860-486-4331 (fax)
>
> --
> __
>
> Vytas Bindokas, Ph.D.
> Research Assoc. / Assoc. Prof.,
> Director, BSD Light Microscopy Core Facility
> Dept Neurobiol Pharmacol Physiol MC0926
> 947 E 58th Street
> The University of Chicago
> Chicago IL 60637
> Room Abbott 120
> 773-702-4875
>
> email
[hidden email]
> web site for LMCF:
>
http://digital.bsd.uchicago.edu/index.html
>
>
>
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