Posted by
Tony Collins-4 on
Oct 25, 2007; 2:17pm
URL: http://imagej.273.s1.nabble.com/3D-reconstruction-of-volume-tp3698102p3698111.html
Hi David,
You may just want to show a single slice from the stack showing the
section of the cell with holes and also show an 'axial' section through
it (draw line; Menu:image>stacks>reslice).
Something like this:
http://www.macbiophotonics.ca/images/axialsection.jpg(CAAX-GFP and SNARF).
Best,
Tony
Tony J. Collins, Ph.D.
McMaster Biophotonics Facility
Dept. Biochemistry and Biomedical Sciences HSC 4H21A
McMaster University, Hamilton, ON, L8N 3Z5
(905) 525 9140 x28812(off.)/x26488(lab)
[hidden email] www.macbiophotonics.ca
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
> David Knecht-charter
> Sent: October 24, 2007 11:47 PM
> To:
[hidden email]
> Subject: 3D reconstruction of volume
>
> We are trying to make volume projections of some confocal z slices
> through a cell. There is cytoplasmic label and some concentrated
> membrane labeling. There are also large vacuoles and the nucleus
> where there is no label. Is there any way to get those black holes
> in the cytoplasm to show up as defined structures in the
> projections? They are obscured by the cytoplasmic label in a
> standard projection because they are of much lower intensity. If you
> try to project just hte lower intensity values, they are obscured by
> the fact that the extracellular background is equally black. Any
> ideas? Thanks- Dave
>
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)