Re: Opening Images from LSM

Posted by Mario Emmenlauer-3 on Oct 27, 2007; 11:01am
URL: http://imagej.273.s1.nabble.com/Re-Opening-Images-from-LSM-tp3698124.html

Hi,

Oliver Braubach wrote:
> ImageJ says that they are at 16-bit?

well, it would be interesting to see if the lsm is indeed 8, 12 or 16 bit.
A simple test might be the filesize. Take
  x-resolution * y-resolution * slices * stacks,
that should be the rough filesize for 8 bit.
If your filesize is 3/2 of that, its 12 bit.
If your filesize is twice that, its 16 bit.

So for a single stack of 512 * 512 * 20 slices * 2 channels,
you should have 10485760 byte ~ 10MB for 8 bit, 15MB for
12 bit or 20MB for 16 bit.

Of course this only works if you have uncompressed lsm,
but mine are always uncompressed so chances are good.

 > Is there some resampling going on? Do I
> need to change this? Any insight would be most welcome.

If you have 12 or 16 bit, your monitor can only show you
8 bit of that. You can use the Brightness/Contrast setting
to change which 'region' of your dynamic range you want to
see. Or you can make a gamma-correction (0.5 or 0.4 should
be fine) to compress the dynamic range, then it will 'fit'
on your monitor.

Cheers,
   Mario

--
Mario Emmenlauer                             Phone: [phone]
Institute for Computer Science               Fax:   [phone]
Chair of Pattern Recognition and Image Processing
Albert-Ludwigs-University
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79110 Freiburg             mailto:emmenlau * informatik.uni-freiburg.de
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