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Re: Opening Images from LSM

Posted by Michael Doube-2 on Oct 27, 2007; 11:12am
URL: http://imagej.273.s1.nabble.com/Re-Opening-Images-from-LSM-tp3698124p3698125.html

Hi Oliver.  Open one of your images, hit [h] to get a histogram - the  
values should be spread out across the full range with a peak or two  
depending on the subject.  Then open image-> adjust-> brightness and  
contrast and adjust the max and min values to be just above and below  
your image data. hopefully this will make a difference- it's possible  
that imageJ's default windowing isn't working well for your images.

Good luck

Mike

Quoting Oliver Braubach <[hidden email]>:

> Hi
>
> I am having trouble viewing and processing Confocal Stacks (in LSM format,
> usually 8-bit) in ImageJ. The problem is that through being opened  
> with ImageJ
> individual slices in the stack become artificiallly overexposed, leaving them
> as nothing more but a lot of white and black - all the detail (gray) is lost.
> The images are fine when viewed in LSM image browser (Zeiss) and are  
>  not badly
> taken.
> ImageJ says that they are at 16-bit? Is there some resampling going on? Do I
> need to change this? Any insight would be most welcome.
> Thanks.
> Oliver
>