Re: Opening Images from LSM
Posted by
Michael Doube-2 on
Oct 27, 2007; 11:12am
URL: http://imagej.273.s1.nabble.com/Re-Opening-Images-from-LSM-tp3698124p3698125.html
Hi Oliver. Open one of your images, hit [h] to get a histogram - the
values should be spread out across the full range with a peak or two
depending on the subject. Then open image-> adjust-> brightness and
contrast and adjust the max and min values to be just above and below
your image data. hopefully this will make a difference- it's possible
that imageJ's default windowing isn't working well for your images.
Good luck
Mike
Quoting Oliver Braubach <
[hidden email]>:
> Hi
>
> I am having trouble viewing and processing Confocal Stacks (in LSM format,
> usually 8-bit) in ImageJ. The problem is that through being opened
> with ImageJ
> individual slices in the stack become artificiallly overexposed, leaving them
> as nothing more but a lot of white and black - all the detail (gray) is lost.
> The images are fine when viewed in LSM image browser (Zeiss) and are
> not badly
> taken.
> ImageJ says that they are at 16-bit? Is there some resampling going on? Do I
> need to change this? Any insight would be most welcome.
> Thanks.
> Oliver
>