Re: Opening Images from LSM

Posted by OliverB on Oct 29, 2007; 3:01pm
URL: http://imagej.273.s1.nabble.com/Re-Opening-Images-from-LSM-tp3698124p3698126.html

Dear Michael

Thanks for you input regarding my ImageJ troubles.
I followed your suggestion and was able to overcome this problem.

Greetings from Canada
Oliver Braubach

Laboratory of Synaptic Function and Plasticity
Department of Physiology and Biophysics
Dalhousie University
Halifax, NS B3H 1X5 Canada
Tel: [phone]
Fax: [phone]




On Oct 27, 2007, at 7:12 AM, Michael Doube wrote:

> Hi Oliver.  Open one of your images, hit [h] to get a histogram -  
> the values should be spread out across the full range with a peak  
> or two depending on the subject.  Then open image-> adjust->  
> brightness and contrast and adjust the max and min values to be  
> just above and below your image data. hopefully this will make a  
> difference- it's possible that imageJ's default windowing isn't  
> working well for your images.
>
> Good luck
>
> Mike
>
> Quoting Oliver Braubach <[hidden email]>:
>
>> Hi
>>
>> I am having trouble viewing and processing Confocal Stacks (in LSM  
>> format,
>> usually 8-bit) in ImageJ. The problem is that through being  
>> opened  with ImageJ
>> individual slices in the stack become artificiallly overexposed,  
>> leaving them
>> as nothing more but a lot of white and black - all the detail  
>> (gray) is lost.
>> The images are fine when viewed in LSM image browser (Zeiss) and  
>> are  not badly
>> taken.
>> ImageJ says that they are at 16-bit? Is there some resampling  
>> going on? Do I
>> need to change this? Any insight would be most welcome.
>> Thanks.
>> Oliver
>>