Posted by
your name Charles Lessman on
Oct 26, 2007; 4:19pm
URL: http://imagej.273.s1.nabble.com/FFT-along-line-in-image-tp3698130p3698135.html
I too can give a testimonial to the value of flatbed scanners in imaging large arrays (in my case zebrafish embryos or oocytes). We use transparency adapters to image time lapse sequences of live embryos and oocytes (for those interested see the refs below). Most recently we have used UMAX Astra 6700 (cost ~$100 !!!) to produce 1200dpi images that are then stacked and analyzed with ImageJ. The scanners are particularly good for getting an overview of many simultaneous treatments and may be automated with macros to scan & archive files unattended. My 2 cents.
39) Lessman, C.A. (2002) Use of computer-aided screening (CAS) for detection of motility mutants in zebrafish embryos. Real-Time Imaging 8:189-201.
41) Lessman, C.A. (2004) Computer-Aided Screening for Zebrafish Embryonic Motility Mutants. Methods in Cell Biology. 76:285-313.
42) Lessman, C. A., Nathani, R., Uddin, R., Walker, J. and Liu, J. (2007). Computer-aided meiotic maturation assay (CAMMA) of zebrafish (Danio rerio) oocytes in vitro. Molecular Reproduction and Development 74:99-109.
http://dx.doi.org/10.1002/mrd.20530Charles A. Lessman, Ph.D.
Professor
Dept. of Biology
The University of Memphis
Memphis, TN 38152
(901) 678-2963
FAX (901) 678-4457
http://biology.memphis.edu/clessman.htmhttp://umpeople.memphis.edu/clessman-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Jim Quinn
Sent: Friday, October 26, 2007 9:25 AM
To:
[hidden email]
Subject: Re: OT - Using film or flatbed scanners for microscope slide scanning
Gabriel and Folks
A good scanner, such as an Epson V700,
will give you surprisingly great results
from about 1x to 30x. You can scan far
greater areas than can be viewed under
the typical stereoscope. I routinely
collect 50MByte images, with full control
over brightness, contrast, gamma, colours, etc....
Try collecting a 2"x2" area at 2400 dpi
on a StereoZoom4 at 30X. It is a snap on
a V700 (and other scanners). The image is
about 65MByte. Then try 4"x4" or 8"x10".
No camera has that many equivalent megapixels.
The only down side is the very limited
control of the lighting direction.
regards,
JQuinn
PS: This thread is better off on the MSA listserver,
the Yahoo group on Microscopy, or the Usenet
group on Microscopy.
> From
[hidden email] Thu Oct 25 17:32:26 2007
> Message-ID: <
[hidden email]>
> Date: Thu, 25 Oct 2007 22:35:20 +0100
> Reply-To: ImageJ Interest Group <
[hidden email]>
> Sender: ImageJ Interest Group <
[hidden email]>
> From: Gabriel Landini <
[hidden email]>
> Organization: The University of Birmingham, UK.
> Subject: Re: OT - Using film or flatbed scanners for microscope slide scanning
> To:
[hidden email]
> In-Reply-To: <
[hidden email]>
> Precedence: list
>
> On Thursday 25 October 2007, Nicholas Verge wrote:
> > For my purposes i dont need magnifaction beyond the equivalent of a that
> > provided by a x10 microscope objective as the mineral grains in my rocks
> > samples are quite coarse. I need to obtain nice evenly illuminated sharp
> > images of the entire thinsection if possible.
>
> I have no idea of costs, but the advantage of a microscope is that you can
> change magnifications on the fly, add filters, use different cameras, and
> illumination settings.
> If you never used a scanner before, be aware that you may be disappointed by
> the lack of visual feedback between inserting a slide and seeing what has
> been scanned. You will not be able to do lapse imaging nor do average capture
> to reduce random noise.
>
> Before buying make sure that you have a demo to avoid disappointments.
> Cheers,
>
> G.
>