Densitometry

Posted by Van Hoomissen, Jacqueline on Oct 16, 2007; 6:33pm
URL: http://imagej.273.s1.nabble.com/Densitometry-tp3698210.html

Greetings,

 

I'm new to ImageJ and I have one, probably very simple question.

 

Does anyone know how to change the "direction" of the unsigned integers
(0 to 65, 535) when using Image J for densitometry of x-ray films?

 

I've scanned my x-ray film using a Microtek Scan Maker 1000XL scanner as
a 16-bit grayscale image (saved as a TIFF file) and then imported the
images into the IMAGE J software.  

 

Now when I analyze the sections the "darker" images on the film are
being assigned low integers and the "lighter" images on the film are
reading higher integers. I'd like IMAGE J to convert this so that the
darker the image the higher the integer assigned.

 

 

Thanks for your help

 

Jacqueline D. Van Hoomissen, Ph.D.

Department of Biology

University of Portland

5000 North Willamette Boulevard

Portland, Oregon 97203 USA

503-943-7779 (Office)

503-943-7123 (Science Office)

503-943-7784 (Fax)

[hidden email]

www.up.edu