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Re: Densitometry

Posted by Joel Sheffield on Oct 16, 2007; 8:33pm
URL: http://imagej.273.s1.nabble.com/Densitometry-tp3698210p3698213.html

This, in fact will not change the values of the pixels, just the
appearance.  What you need to do us use Edit>Invert to change the
values so that they go in the right direction.

Joel

Date sent:       Tue, 16 Oct 2007 14:01:10 -0400
Send reply to:   ImageJ Interest Group <[hidden email]>
From:           David Hovis <[hidden email]>
Subject:         Re: Densitometry
To:             [hidden email]

> Image -> Lookup Tables -> Invert LUT  should do what you want.
>
> --David
>
> ----------------------------------------
> David Hovis
> Senior Research Associate
> Department of Materials Science
> Case Western Reserve University
> [hidden email]
>
> On Oct 16, 2007, at 1:33 PM, Van Hoomissen, Jacquie wrote:
>
> > Greetings,
> >
> >
> >
> > I'm new to ImageJ and I have one, probably very simple question.
> >
> >
> >
> > Does anyone know how to change the "direction" of the unsigned  
> > integers
> > (0 to 65, 535) when using Image J for densitometry of x-ray films?
> >
> >
> >
> > I've scanned my x-ray film using a Microtek Scan Maker 1000XL  
> > scanner as
> > a 16-bit grayscale image (saved as a TIFF file) and then imported the
> > images into the IMAGE J software.
> >
> >
> >
> > Now when I analyze the sections the "darker" images on the film are
> > being assigned low integers and the "lighter" images on the film are
> > reading higher integers. I'd like IMAGE J to convert this so that the
> > darker the image the higher the integer assigned.
> >
> >
> >
> >
> >
> > Thanks for your help
> >
> >
> >
> > Jacqueline D. Van Hoomissen, Ph.D.
> >
> > Department of Biology
> >
> > University of Portland
> >
> > 5000 North Willamette Boulevard
> >
> > Portland, Oregon 97203 USA
> >
> > 503-943-7779 (Office)
> >
> > 503-943-7123 (Science Office)
> >
> > 503-943-7784 (Fax)
> >
> > [hidden email]
> >
> > www.up.edu
> >
> >


--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
[hidden email]  
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs