measuring colors-heterogenous areas

Posted by Attin, Mina on Oct 09, 2007; 6:44pm
URL: http://imagej.273.s1.nabble.com/measuring-colors-heterogenous-areas-tp3698231.html

Hi,
I recently started to use ImageJ to quantify the staining (blue color) of
different heart tissue segments. I use a method that does not allow me to
quantify the % of staining in each segment accurately because the staining
(blue color) is very heterogeneous. The color of heart tissue is dark pink
and the staining is the blue color that is distributed heterogeneously
throughout each segment. I will explain my method and I will appreciate if
you have any suggestion to improve the method or suggest different way to
quantify the blue color. For each image, I start with image/color/RBG
split, then I use plugins to use filter/multithreshold. I try to determine
a reasonable threshold (the red color to cover the staining only-this is
hard because the staining is very heterogenous) and I measure the area.
Finally, I measure the total area of each segment and I will obtain the %
of staining by dividing the staining area to the whole area.

Best regards,

M