ROI selection in line scan pictures

Posted by Alexandre Santos-4 on Oct 04, 2007; 4:09pm
URL: http://imagej.273.s1.nabble.com/ROI-selection-in-line-scan-pictures-tp3698271.html

Hello,

I would like to analyze some confocal line scan pictures I acquired in
two channels. Each channel (called z slice in imagej) is a picture of
512 pixels width and 50,000 pixels height

I would like to be able to select a segment of the line scan, and
obtain average intensity levels for each line of the scan, in both
channels.

At the moment I have two difficulties:

1 - I can't find a way to select a segment as a ROI. If I want to see
the picture, I need to magnify it a lot, but then I can't draw a
rectangle encompassing the whole picture.

2 - Once I have the desired rectangle, How do I get average intensity
values for each line of the rectangle, and not for the whole
rectangle? (I thought about using the Gel analysis tool, but I don't
know if that would work)

Cheers,

Alexandre Santos
Neuro-MPI, Martinsried, Germany