Posted by
Harry Parker on
Oct 05, 2007; 4:31am
URL: http://imagej.273.s1.nabble.com/ROI-selection-in-line-scan-pictures-tp3698271p3698272.html
Hello Alexandre,
Check out the menu command, Edit->Selection->Specify. There you can define the region numerically. You can make the rectangle 1 line high to get a single scan line to average. You can use the arrow keys to move one row at a time. The Option key with the arrow keys lets you shrink or grow the size of the ROI. You can write a simple macro to read your selection limits and loop through the lines, measuring each one.
--
Harry Parker
Senior Systems Engineer
Digital Imaging Systems, Inc.
----- Original Message ----
From: Alexandre Santos <
[hidden email]>
To:
[hidden email]
Sent: Thursday, October 4, 2007 11:09:15 AM
Subject: ROI selection in line scan pictures
Hello,
I would like to analyze some confocal line scan pictures I acquired in
two channels. Each channel (called z slice in imagej) is a picture of
512 pixels width and 50,000 pixels height
I would like to be able to select a segment of the line scan, and
obtain average intensity levels for each line of the scan, in both
channels.
At the moment I have two difficulties:
1 - I can't find a way to select a segment as a ROI. If I want to see
the picture, I need to magnify it a lot, but then I can't draw a
rectangle encompassing the whole picture.
2 - Once I have the desired rectangle, How do I get average intensity
values for each line of the rectangle, and not for the whole
rectangle? (I thought about using the Gel analysis tool, but I don't
know if that would work)
Cheers,
Alexandre Santos
Neuro-MPI, Martinsried, Germany
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