http://imagej.273.s1.nabble.com/ROI-selection-in-line-scan-pictures-tp3698271p3698273.html
I would like to thank Wayne, John and Harry for their answers. I am
> Hello Alexandre,
> Check out the menu command, Edit->Selection->Specify. There you can define the region numerically. You can make the rectangle 1 line high to get a single scan line to average. You can use the arrow keys to move one row at a time. The Option key with the arrow keys lets you shrink or grow the size of the ROI. You can write a simple macro to read your selection limits and loop through the lines, measuring each one.
>
> --
> Harry Parker
> Senior Systems Engineer
> Digital Imaging Systems, Inc.
>
> ----- Original Message ----
> From: Alexandre Santos <
[hidden email]>
> To:
[hidden email]
> Sent: Thursday, October 4, 2007 11:09:15 AM
> Subject: ROI selection in line scan pictures
>
> Hello,
>
> I would like to analyze some confocal line scan pictures I acquired in
> two channels. Each channel (called z slice in imagej) is a picture of
> 512 pixels width and 50,000 pixels height
>
> I would like to be able to select a segment of the line scan, and
> obtain average intensity levels for each line of the scan, in both
> channels.
>
> At the moment I have two difficulties:
>
> 1 - I can't find a way to select a segment as a ROI. If I want to see
> the picture, I need to magnify it a lot, but then I can't draw a
> rectangle encompassing the whole picture.
>
> 2 - Once I have the desired rectangle, How do I get average intensity
> values for each line of the rectangle, and not for the whole
> rectangle? (I thought about using the Gel analysis tool, but I don't
> know if that would work)
>
> Cheers,
>
> Alexandre Santos
> Neuro-MPI, Martinsried, Germany
>
>
>
>
>
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