Re: fluorescence intensity

Posted by Michael Weber-4 on Sep 24, 2007; 10:54am
URL: http://imagej.273.s1.nabble.com/fluorescence-intensity-tp3698342p3698343.html

Hi Anette,

the are a couple of ways to measure intensity. The most simple way is to
load an image, point the mouse to the pixel of interest and check the
signal intensity in the main tool bar. This could be a first test if the
images where acquired in the range of the detector (=if nothing it
overexposed). Did you acquire all images under the same condition
(excitation power, filter setup, exposure time)? Otherwise it will be
tricky to compare them.

You can also measure regions by drawing i.e. a circle in the cell and go
for "Analyze" > "Measurements". There are also plugins/macros to automate
this, but a couple of manual steps before are probably go to find the best
way.

cheers,
Michael

> Dear ImageJ,
> I have a question how to use the ImageJ program. I have labeled cells with
> a fluorescence dye. I let the cells in culture about several days. Than I
> made pictures with the fluorescence microscope on day 1, 3 and 5 after
> staining. Now I want to messure and compare the fluorescence intensity
> signal over these days and furthermore I want to examine whether there is
> any weakening.
> So, can you say me how to to this with the ImageJ program?
>
> Thank you.
>
> With kind regards,
> Anette Christ