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Re: Q: Definition of densitometry (probably very academic)

Posted by Michael Cammer on Sep 05, 2007; 2:48am
URL: http://imagej.273.s1.nabble.com/Replace-one-value-by-NaN-tp3698434p3698438.html

The real experts may step in to correct me or to elaborate, but
essentially measuring fluorescence is a linear relationship between # of
molecules and intensity.  However, densitometry is the measurement of
absorbence by a density and/or thickness of material and this is a natural
log function that is conventionally expressed as a log10 function.  e.g.
http://www.aecom.yu.edu/aif/instructions/AGFA_scanner/scan_cal.htm
-Michael



> Dear experts,
>
> I am in some sort of dispute about the correct definition of
> "densitometry" and need your help:
>
> Mine is: When you have e.g. some spots in an image (talking about western
> blots) and you use ImageJ for quantification, then what you are doing is
> densitometry as scanning / recording an image (which actually is
> photometry) and then measuring the intensity of a spot is nothing but
> combining lots of densitometric measurements into a result.
>
> The other opinion is that densitometry only refers do direct analysis e.g.
> of an xray film (where the spots are on) or a membrane which carries some
> fluorescent spots, by a device (sort of scanner is allowed) which directly
> delivers you a figure representing the optical density at a certain
> position / of a certain spot you have marked on a screen before.
>
> I think that this opinion is missing is the fact that any scanned image
> actually is an array of photometric data just waiting to be analyzed by
> densitometry, maybe just on another device / with another software.
>
> What do you think?
>
> Many thanks!
>
> Wo
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_________________________________________
Michael Cammer   http://www.aecom.yu.edu/aif/