Posted by
Anne Salomon on
Aug 19, 2007; 12:20am
URL: http://imagej.273.s1.nabble.com/calibrat-to-known-function-tp3698569p3698571.html
Thanks for your detailed and speedy reply Harry! Unfortunately, my problem
is still not solved. Here's why...
I have made a .lut file just as you said BUT when I use it in ImageJ, the
1-255 pixels, although defined in color, are not defined in the value of the
chlorophyll a they should represent i.e. pixel =(log10 (chla)+2)/0.015.So,
when I make my image the colors are not calibrated to the function above.
So, how do I assign each pixel (that now has the correct color based on my
.lut file) a value of chl a given the above equation?
Thanks again for your help,
Anne
----- Original Message -----
From: "Harry Parker" <
[hidden email]>
To: <
[hidden email]>
Sent: Saturday, August 18, 2007 2:05 PM
Subject: Re: calibrat to known function
> Hi Anne,
>
> An ImageJ LUT can be defined simply as a text file, 257 rows by 4 columns.
> The 1st row contains text. Each following row has 4 integers from 0 to
> 255, separated by tabs: index, red, green, and blue values. Index is
> incremented from 0 to 255. Then for each index give the values wanted for
> red, green and blue. If you want grey values, red, green and blue are set
> equal to each other.
>
> I created something similar using Excel to calculate the LUT numbers.
>
> Rename the file with the extension, ".lut", and place it in a folder named
> "LUTs" in the ImageJ folder.
>
> --
> Harry Parker
> Senior Systems Engineer
> Digital Imaging Systems, Inc.
>
> ----- Original Message ----
> From: Anne Salomon <
[hidden email]>
> To:
[hidden email]
> Sent: Saturday, August 18, 2007 3:44:43 PM
> Subject: calibrat to known function
>
> I am trying to calibrate pixel colors 1-255 of a LUT to a known function:
> pixel=((log x+2))/0.15. How do I do this in image J?
>
> Thanks very much for you insight,
> Anne
>
>
>
>
>
>
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