particle tracking

Posted by Kuljis, Dika Ana on Jul 24, 2007; 7:47pm
URL: http://imagej.273.s1.nabble.com/particle-tracking-tp3698762.html

I am using ImageJ to measure areas of axons and their surrounding myelin
sheathes. ImageJ reports each "particle" area which is in my situation either
the axon or the myelin and are similar to a donut and a donut-hole shape. I need
to be able to attribute each donut-hole (the axon) to its surrounding donut
(myelin), but do not know if there is any way to label, track, or verify the
pairing.
 
I am working with two images of one picture: one that shows only the donut
shaped myelin, and one that shows the donut-hole shaped center axon. As was
explained to me, area assessment is ordinal from the top left of the image and
goes downward. However, the location of my particles isn't always so clear cut.
For instance, the myelin may be higher in one pass then it would be for its
respective axon and therefore, might be attributed to an axon not its own. These
are irregular shapes and sometimes thicker or thinner, and so the order will be
flip flopped and the order of my axon image will not correspond to the order of
my myelin image.
 
Is there some feature available that will help me track which particle is being
assigned which number? Are there any suggestions as tho how I can use ImageJ to
get around this problem?
 
 





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