Re: particle tracking

Posted by Michael Cammer on Jul 25, 2007; 2:41pm
URL: http://imagej.273.s1.nabble.com/particle-tracking-tp3698762p3698764.html

We had the exact same problem with measuring dark myelin around light
axons.  The way we dealt with it was to segment out the myelin
rings.  Then, for each extracted region of interest  duplicate the
image, clear outside the myelin and then do the measurements within
the region of interest.   We stored the results in our own arrays and
printed them out in a tabbed table at the end. This solved the
problems of centroids of myelin that did not fall neatly within the
axons and guaranteed a one-to-one relationship in the measurements.

____________________________________________________________________________
Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of Med.
URL:  http://www.aecom.yu.edu/aif/