Re: corelation between pixel value to real scale

Posted by sharmistha paul on
URL: http://imagej.273.s1.nabble.com/corelation-between-pixel-value-to-real-scale-tp3698820p3698821.html

Hi Prof. Lessman,

Thank you for your response.
Could you tell me please what is the pixel aspect ratio? Is it the ratio of two values shown by cursor when I move it on picture?
Regards,

Sharmistha


----- Original Message ----
From: Charles A Lessman (clessman) <[hidden email]>
To: [hidden email]
Sent: Wednesday, July 18, 2007 7:53:40 PM
Subject: Re: corelation between pixel value to real scale


With image open, select set scale from analyze menu and fill in drop
down. Then redo your calculations.

Charles A. Lessman, Ph.D.
Professor
Dept. of Biology
The University of Memphis
Memphis, TN 38152
[phone]
FAX [phone]
http://biology.memphis.edu/clessman.htm
http://umpeople.memphis.edu/clessman

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
sharmistha paul
Sent: Wednesday, July 18, 2007 1:27 PM
To: [hidden email]
Subject: corelation between pixel value to real scale

Hi everybody,

I've 8 bit SEM image. I calculated surface roughness using plugin. But
it gives Rq value in pixel. How can I corelate this to real scale (
micrometer) ?
Please give some advice.

Cheers

Sharmistha



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