SV: corelation between pixel value to real scale

Posted by Gary Chinga on
URL: http://imagej.273.s1.nabble.com/corelation-between-pixel-value-to-real-scale-tp3698820p3698823.html

I assume that if you have 8-bit SEM images then you are just calculating the greylevel variation of the images, not the real roughness values. For calculating the roughness (e.g. Rq) you need to reconstruct the surface, using e.g. SEM stereo imaging.

The Analyze/Set scale... will only calibrate the x,y dimensions, not the heights (z).

Gary.
http://www.gcsca.net 

>
>
> > From: Charles A Lessman (clessman) [[hidden email]]
> > Sent: 2007-07-18 20:53:40 CEST
> > To: [hidden email]
> > Subject: Re: corelation between pixel value to real scale
> >
> > With image open, select set scale from analyze menu and fill in drop
> > down. Then redo your calculations.
> >
> > Charles A. Lessman, Ph.D.
> > Professor
> > Dept. of Biology
> > The University of Memphis
> > Memphis, TN 38152
> > [phone]
> > FAX [phone]
> > http://biology.memphis.edu/clessman.htm
> > http://umpeople.memphis.edu/clessman
> >  
> > -----Original Message-----
> > From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> > sharmistha paul
> > Sent: Wednesday, July 18, 2007 1:27 PM
> > To: [hidden email]
> > Subject: corelation between pixel value to real scale
> >
> > Hi everybody,
> >
> > I've 8 bit SEM image. I calculated surface roughness using plugin. But
> > it gives Rq value in pixel. How can I corelate this to real scale (
> > micrometer) ?
> > Please give some advice.
> >
> > Cheers
> >
> > Sharmistha
> >
> >
> >  
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