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area measurement problems

Posted by J.C. Steele on Jul 12, 2007; 8:02pm
URL: http://imagej.273.s1.nabble.com/area-measurement-problems-tp3698872.html

Hello all,

  I am helping a friend of mine on the technical aspects of measuring the
area for a project she is working on.  I am having some problems with the
measurement though....what we have is a collection of photos (about 900) of
mussel's and need the measure the overall area of the shell, and then the
area of the damaged portion of the shell (the white portion in the picture
referenced below).  I have been using the tutorial at
http://rsb.info.nih.gov/ij/docs/pdfs/examples.pdf to help me measure the
area, however I have ran into some problems I need some assistance with.
First off, most (if not all)  of the specimens collected have additional
"material" attached the shells that i believe is being included in the
measurements...mine example is at
https://ww2.coastal.edu/jcsteele/imagej/10A.JPG which on the bottom side of
the shell you can see some grasses, etc.  Is there a way to remove this from
the image, or exclude it from the measurement?  As you can see from
https://ww2.coastal.edu/jcsteele/imagej/make_binary.jpg and
https://ww2.coastal.edu/jcsteele/imagej/show_outline.jpg when I issue the
"make binary" command from the tutorial, alot of a of the excess material is
being thrown into the mix when calculating the area (I believe).  Any ideas
on where i should start?

Just for the sake of telling everyone, I do not have much experience with
imagej, however my friend was using it and using the selection tool to
select each individual pixel around the shell, then around the damaged
portion of the shell to make a measurement....this is painfully long, and
with as many photos as she has would take along time...this is when I found
the tutorial (before she was not setting a measurement scale or anything
which I believe would cause severe problems and told her...this is when she
asked me for assistance.)

Any help or comments at all would be appreciated and welcomed, Thanks.


Josh