Re: Deconvolve 3D plugin problem

Posted by Przemko on Jul 05, 2007; 10:20am
URL: http://imagej.273.s1.nabble.com/Workflow-for-importing-DV-video-tp3698931p3698933.html

Gentlemen, thank you for the prompt response. Here is some more info
about my images and what happens. So, first of all I am not doing a 3D
medical imaging but immunofluorescence. Normally I use the Iterative
Deconvolve plugin and it works on my images rather well. I have decided
however to give it a try with Deconvolve 3D because some of my images
would not be processed to the point that I was happy with them. Namely,
I am looking at the colocalization of two proteins and sometimes, with
insufficient deconvolution, I had troubles deciding if the two proteins
colocalized or not.
My images are 1315x1033 pixels. If I do Diffraction Limit PSF and then
Iterative Deconvolution, the rpogram works and on my machine it takes
about 60 seconds to 5 rounds of deconvolution. Now if I go with the same
file to Diffraction PSF 3D and Iterative Deconvolution 3D, I get the
crash as described before. If I decrease the number of pixel by half
(loosing some of the resolution which I need), it seems that the program
goes a bit longer (2-3 min) before crashing as mentioned earlier. The
reason to go for the 3D plugin comes from the info on the plugin site
stating "This plugin is intended to replace both the Iterative
Deconvolution and Iterative Deconvolution 2 plugins for 2D applications,
as well as extending the function to 3D"

So, here I am. What I wonder is, as one of you suggested, so do the
processing in parts so that the memory usage is better controlled. Would
that be somehow possible?

Again, thank you for your help!

Przemko

--
        Przemko Tylzanowski Ph.D.
        LSD & Joint, box 813-GREEN
        O & N
        University of Leuven
        Herestraat 49
        3000 Leuven
        Belgium
       
        phone: [phone]
        fax  : [phone]
       




--
         Przemko Tylzanowski Ph.D.
         LSD & Joint, box 813-GREEN
         O & N
         University of Leuven
         Herestraat 49
         3000 Leuven
         Belgium
         
         phone: [phone]
         fax  : [phone]
         


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