Densitometry guide (lines)?

Posted by Wolfgang Schechinger on Jun 28, 2007; 9:40am
URL: http://imagej.273.s1.nabble.com/Densitometry-guide-lines-tp3698971.html

Dear IJ experts,

we use ImageJ for the densitometry of western blots (ctrl 1/2/3
sequece), raw data is generated by an infrared fluorescence scanner
(Licor Odyssey). Are there any guidelines how to define peaks and to
integrate them, esp. when there is no baseline separation of bands or
the baseline is sort of skew?

Are there sort of "official rules", articles one should know or a
good book (chapter) on the topic?
Googling was not much successful for me yet.

Many thanks and best regards,

Wolfgang