Modifying Image Histograms

Posted by Rotella, Anthony M. (GRC)[] on Jun 21, 2007; 4:27pm
URL: http://imagej.273.s1.nabble.com/Modifying-Image-Histograms-tp3698976.html

Hello,

        I need to threshold a large number of images that are similar,
but not quite the same. The vast majority threshold satisfactorily
within the same range, but some are far enough off to throw the
calculations and the accuracy out the window. Basically, I was wondering
if there is a way to change the images so that the important features of
the histograms of the slices in the stack fit into the same range, but
so that the pixels still maintain their relative values between each
other, so the same features of the image still threshold out correctly.
Unfortunately the Autothreshold is way off. I tried Enhance Contrast,
but makes the images very messy and doesn't work well. Another fix to my
problem is if someone knows how to increase the difference in pixel
intensities between objects in the foreground and background. I can
clearly see the difference , but of course, ImageJ can't just do what I
want, and some of the objects that I want to have thresholded out have
the same intensities as something in the background, maybe somewhere
else in the image. Unfortunately, I am hesitant to post the images on a
hosting service, but I can e-mail them to anyone that wants to see them.
Thanks, and sorry for the vague question!

Tony Rotella