http://imagej.273.s1.nabble.com/Modifying-Image-Histograms-tp3698976p3698980.html
histogram matching (or specification) IS available for ImageJ in the source code section of our book's support site at www.imagingbook.com . While the English edition is scheduled to appear in September, you could for now download the source code for the German edition from
which contains English comments anyway. Hope this helps.
Dr. Wilhelm BURGER
| upper austria univ. of applied sciences
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On
> Behalf Of Giancarlo Mascetti
> Sent: Thursday, June 21, 2007 7:01 PM
> To:
[hidden email]
> Subject: Re: Modifying Image Histograms
>
> Hello Tony,
> A method called "histogram matching" probably could
> solve your
> problem (the use the same threshold for a large number of "similar"
> images)... but unfortunately it is not yet available in
> ImageJ (or as a plugin)
> Some years ago I have written a matlab routine to perform
> such task but was
> very slow...
>
> Giancarlo
>
> At 10.27 21/06/07 -0500, you wrote:
> >Hello,
> >
> > I need to threshold a large number of images that
> are similar,
> >but not quite the same. The vast majority threshold satisfactorily
> >within the same range, but some are far enough off to throw the
> >calculations and the accuracy out the window. Basically, I
> was wondering
> >if there is a way to change the images so that the important
> features of
> >the histograms of the slices in the stack fit into the same
> range, but
> >so that the pixels still maintain their relative values between each
> >other, so the same features of the image still threshold out
> correctly.
> >Unfortunately the Autothreshold is way off. I tried Enhance Contrast,
> >but makes the images very messy and doesn't work well.
> Another fix to my
> >problem is if someone knows how to increase the difference in pixel
> >intensities between objects in the foreground and background. I can
> >clearly see the difference , but of course, ImageJ can't
> just do what I
> >want, and some of the objects that I want to have
> thresholded out have
> >the same intensities as something in the background, maybe somewhere
> >else in the image. Unfortunately, I am hesitant to post the
> images on a
> >hosting service, but I can e-mail them to anyone that wants
> to see them.
> >Thanks, and sorry for the vague question!
> >
> >Tony Rotella
>
> Giancarlo Mascetti, Ph.D.
> X-Istituto di Calcolo Scientifico
>
> c/o Paramed Health Services Ltd.
> c.so F. M. Perrone, 73r - 16152 Genova, Italy
> tel. 010-6489265 - mob. 335-7787927 - fax. 010-7404530
> E-mail:
[hidden email]
>