Posted by
Giancarlo Mascetti on
Jun 21, 2007; 6:01pm
URL: http://imagej.273.s1.nabble.com/Modifying-Image-Histograms-tp3698976p3698982.html
Hello Tony,
A method called "histogram matching" probably could solve your
problem (the use the same threshold for a large number of "similar"
images)... but unfortunately it is not yet available in ImageJ (or as a plugin)
Some years ago I have written a matlab routine to perform such task but was
very slow...
Giancarlo
At 10.27 21/06/07 -0500, you wrote:
>Hello,
>
> I need to threshold a large number of images that are similar,
>but not quite the same. The vast majority threshold satisfactorily
>within the same range, but some are far enough off to throw the
>calculations and the accuracy out the window. Basically, I was wondering
>if there is a way to change the images so that the important features of
>the histograms of the slices in the stack fit into the same range, but
>so that the pixels still maintain their relative values between each
>other, so the same features of the image still threshold out correctly.
>Unfortunately the Autothreshold is way off. I tried Enhance Contrast,
>but makes the images very messy and doesn't work well. Another fix to my
>problem is if someone knows how to increase the difference in pixel
>intensities between objects in the foreground and background. I can
>clearly see the difference , but of course, ImageJ can't just do what I
>want, and some of the objects that I want to have thresholded out have
>the same intensities as something in the background, maybe somewhere
>else in the image. Unfortunately, I am hesitant to post the images on a
>hosting service, but I can e-mail them to anyone that wants to see them.
>Thanks, and sorry for the vague question!
>
>Tony Rotella
Giancarlo Mascetti, Ph.D.
X-Istituto di Calcolo Scientifico
c/o Paramed Health Services Ltd.
c.so F. M. Perrone, 73r - 16152 Genova, Italy
tel. 010-6489265 - mob. 335-7787927 - fax. 010-7404530
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