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Re: particle distribution - randomness/uniformity

Posted by Kenneth Sloan-2 on May 25, 2007; 8:27pm
URL: http://imagej.273.s1.nabble.com/particle-distribution-randomness-uniformity-tp3699301p3699305.html

On May 25, 2007, at 10:54 AM, Albert Cardona wrote:

> Statistics books suggest that a random spread of particles would  
> follow a Poisson distribution.

This is not realistic for points which are representative centers of  
finite-sized particles.  There are two common twiddles: a HARD center  
(where a given point absolutely forbids another point with a small  
epsilon, and a SOFT center (where the observed density rises as you  
move away from a particular central point.  Variations on these  
schemes were all the rage 20 years ago.  They are based on real  
physical considerations on where a "point" can actually be.

Just about the most complicated distribution worth worrying about has  
a HARD region  (R0) where other points are excluded, followed by a  
smooth transition from 0 density to a Maximum density (from R0 to  
R1), followed by  an absolutely uniform distribution at distances  
greater than R1.


> For example, consider as many cells in a grid over the image as  
> particles per image. Then, following a Poisson, determine the  
> expected number of cells with zero, one, two, etc. particles.
> Finally compare the distribution of your spread with the expected,  
> Poisson-following one. You can do this with a Chi-square.
>
> The above gives you a qualitative answer: are your particles  
> randomly distributed or not. If they are, then you can do a k-means  
> clustering analysis to find out how many clusters arise from your  
> particle spread.
>

Quite correct - just be careful of physical considerations that make  
0 < R0 < R1.  As a sanity check, if you set R1 = R0 = 0, then you  
should get the pure Poisson Distribution (assuming, with complete  
loss of generality, that no two points are in the IDENTICAL position.

> Albert

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