Re: Plot Profile axis

Posted by Jonathan Hilmer on May 15, 2007; 9:42pm
URL: http://imagej.273.s1.nabble.com/Plot-Profile-axis-tp3699461p3699463.html

Perhaps I don't fully understand your question, but wouldn't it be
possible to make them into a stack, then perform the plotting once for
each layer in the stack?

Keep in mind that regardless how you get the plots, by default their
vertical axes will be different.  That issue has been addressed
previously in the mailing list: search for posts by Schechinger,
Mutterer, and myself to find a few of the discussions.  In particular,
this was brought up by Mutterer and might be just the thing for you,
though I haven't tried it personally:
http://rsbweb.nih.gov/ij/macros/SinglePanelGelAnalyzer.txt


Jonathan Hilmer



On 5/14/07, Chao-Yin Chen <[hidden email]> wrote:

> I have collected LSM images with three different dyes.  The Image J
> opens the file into three separate windows (Red, Green and Blue). I
> would like to be able to see the Plot Profile of the same region in
> each channel (a line across a cell).  Is there a way to make this
> easier than making sure I draw the line in the same XY coordinate in
> each window.  I tried merging the images before performing the Plot
> Profile but can't get the information for individual channels.  Any
> suggestion will be greatly appreciated.
>
> Thanks,
>
> Chao
>
>
> Chao-Yin Chen
> Associate Adjunct Professor
> Department of Pharmacology
> University of California, Davis
> Phone: [phone]
> Fax; 530-752-7710
> e-mail: [hidden email]
> web: http://www.ucdmc.ucdavis.edu/pharmacology