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Re: Plot Profile axis

Posted by Chao-Yin Chen on May 15, 2007; 10:13pm
URL: http://imagej.273.s1.nabble.com/Plot-Profile-axis-tp3699461p3699464.html

Perhaps I did not have the right search words.  I spent a few days
trying to find this on the archive with no success.  Thanks for the
suggestions.  This could work well with a stack of images.  The other
option provided by Jim Quinn (see below) did exactly what I wanted to do.

Use "macro record" to see what coordinates you are
selecting.  Then use a macro with the same coords
on all the images.

Thanks again for the suggestions.

Chao


At 01:42 PM 5/15/2007, you wrote:

>Perhaps I don't fully understand your question, but wouldn't it be
>possible to make them into a stack, then perform the plotting once for
>each layer in the stack?
>
>Keep in mind that regardless how you get the plots, by default their
>vertical axes will be different.  That issue has been addressed
>previously in the mailing list: search for posts by Schechinger,
>Mutterer, and myself to find a few of the discussions.  In particular,
>this was brought up by Mutterer and might be just the thing for you,
>though I haven't tried it personally:
>http://rsbweb.nih.gov/ij/macros/SinglePanelGelAnalyzer.txt
>
>
>Jonathan Hilmer
>
>
>
>On 5/14/07, Chao-Yin Chen <[hidden email]> wrote:
>>I have collected LSM images with three different dyes.  The Image J
>>opens the file into three separate windows (Red, Green and Blue). I
>>would like to be able to see the Plot Profile of the same region in
>>each channel (a line across a cell).  Is there a way to make this
>>easier than making sure I draw the line in the same XY coordinate in
>>each window.  I tried merging the images before performing the Plot
>>Profile but can't get the information for individual channels.  Any
>>suggestion will be greatly appreciated.
>>
>>Thanks,
>>
>>Chao
>>
>>
>>Chao-Yin Chen
>>Associate Adjunct Professor
>>Department of Pharmacology
>>University of California, Davis
>>Phone: 530-754-6458
>>Fax; 530-752-7710
>>e-mail: [hidden email]
>>web: http://www.ucdmc.ucdavis.edu/pharmacology
>
>Chao-Yin Chen
>Associate Adjunct Professor
>Department of Pharmacology
>University of California, Davis
>Phone: 530-754-6458
>Fax; 530-752-7710
>e-mail: [hidden email]
>web: http://www.ucdmc.ucdavis.edu/pharmacology