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Re: How to convert pixels into squaremicrometer

Posted by Michael Schmid on May 03, 2007; 2:15pm
URL: http://imagej.273.s1.nabble.com/How-to-convert-pixels-into-squaremicrometer-tp3699617p3699618.html

Hi Sussi,

you need the image scale in pixels per micrometer, e.g. by
taking a picture of an object with known dimensions.
Then, see the ImageJ documentation,
   http://rsb.info.nih.gov/ij/docs/menus/analyze.html#scale
or
   http://imagejdocu.tudor.lu/imagej-documentation-wiki/gui-commands/ 
set-scale

Michael
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On 3 May 2007, at 14:55, Sussi Rørdam Thomasen wrote:

> Hello everybody...
> I am measuring fatcells for my masterproject, but I don't no how to  
> convert the
> area from pixels into squaremicrometer - the pictures that I have  
> measured is
> x20 magnification. Can anybody please help me?
>
> Sussi Thomasen, Denmark