Re: How to convert pixels into squaremicrometer

Posted by Volker Baecker on May 03, 2007; 2:49pm
URL: http://imagej.273.s1.nabble.com/How-to-convert-pixels-into-squaremicrometer-tp3699617p3699619.html

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Hello Sussi,
if you know the size of one ccd-element (from the description of the
camera) you can calculate the pixel size.

        pixel size = size of one ccd-element / magnification

Volker
Michael Schmid a écrit :

> Hi Sussi,
>
> you need the image scale in pixels per micrometer, e.g. by
> taking a picture of an object with known dimensions.
> Then, see the ImageJ documentation,
>   http://rsb.info.nih.gov/ij/docs/menus/analyze.html#scale
> or
>  
> http://imagejdocu.tudor.lu/imagej-documentation-wiki/gui-commands/set-scale
>
> Michael
> ________________________________________________________________
>
> On 3 May 2007, at 14:55, Sussi Rørdam Thomasen wrote:
>
>> Hello everybody...
>> I am measuring fatcells for my masterproject, but I don't no how to
>> convert the
>> area from pixels into squaremicrometer - the pictures that I have
>> measured is
>> x20 magnification. Can anybody please help me?
>>
>> Sussi Thomasen, Denmark
>
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>
>

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