Posted by
Ryan Garrick on
Apr 24, 2007; 1:49am
URL: http://imagej.273.s1.nabble.com/help-standardizing-images-tp3699688.html
Dear All,
I have a question about whether I can perform image standardization in
ImageJ. I have a series of digital photographs (of an insect), and want to
score some morphological traits. As a first step, I want to use the
graycard I have included in each photo to standardize all images, relative
to a single 'reference' image (i.e. just one of the photos, the choice of
which one is to be used as the reference is arbitrary, because I am only
interested in relative measures of phenotypic variation, not absolute
values).
The spirit of what I want to do is something like this:
(1) select the graycard in the reference image, then select the graycard
in the target image;
(2) use ImageJ to calculate what adjustments need to be made to make the
ROI in the target image match the ROI in the reference image;
(3) apply the calculated adjustment to the entire target image;
(4) repeat the procedure for all remaining target images
I hope to end up with all of the images having been standardized, so that
measurements of phenotypic characters that make us of setting thresholds
to objectively delineate edges are not impacted by slight differences in
illumination of the original digital photographs.
Is this possible with ImageJ, and if not, can you direct me towards
software that will perform this sort of task?
Thanks for your help.
cheers,
Ryan
email:
[hidden email]