Re: StereoView after StackFocuser

Posted by Gabriel Landini on Apr 13, 2007; 10:07am
URL: http://imagej.273.s1.nabble.com/StereoView-after-StackFocuser-tp3699767p3699768.html

On Friday 13 April 2007 02:23:44 NeLaS wrote:
> doing the sequence:
> >Open image sequence
> >Run Extended Depth of Field Plugin (EDF)
> >Run Anaglyph plugin (G.Landini) and mark to generate a stereo image
>
> However, the topology map created by EDF does not seem to reflect the
> stack's resulting topology, but only the single output image's topology.

Maybe you are seeing the results of different methods to decide what is on
focus when you use semi-transparent objects.

These plugins would work perfectly on stacks of images of opaque objects. The
problem with semi-transparent objects (like those cells) is that they can be
on focus at several places in the z plane. The plugins try to maximise some
focus function in the z direction. Depending on the implementation this may
be found at different places.

Just curious, which of the topology maps give you the best focused result?

Can you upload the stack for testing?

> I tried to use Anaglyph plugin with the Height Map, but with no success!
> The resulting image is absolutely distorted. I assigned the output and
> heightmap to 32bit and 8bit, but it made no difference.

> ps: sorry for my ignorance, but is heightmap = topology ?
Yes, although the heightmap is not guaranteed to be meaningful (measure-wise)
unless your stacks are all taken at the same step depth.

Regards

G.