Re: Converting and overlay in Image5D to RGB

Posted by Andrew Vaughan-2 on Apr 03, 2007; 11:37am
URL: http://imagej.273.s1.nabble.com/Converting-and-overlay-in-Image5D-to-RGB-tp3699875p3699876.html

In reply to Joachim's post, I was opening LIF files from a Leica confocal using the Bioformats
importer. The stacks had four fluorescent channels (interleaved) and 30 Z slices. I used the 'Stack
to Image5D' plugin to create an Image5D (swapping the third and fourth dimension over to
compensate for the fact that the channels were interleaved). I used the default colours and
selected 'ovl' to get the overlay. I then selected the 'Image5D to RGB' plugin from the Image5D
item in plugins.

Thanks.

Andrew Vaughan.