Deconvolution, PSF, Dougherty's, Besson's plugins....

Posted by Hariharan Iyer on Mar 16, 2007; 5:38pm
URL: http://imagej.273.s1.nabble.com/Deconvolution-PSF-Dougherty-s-Besson-s-plugins-tp3700007.html

Dear ImageJ users,
I am very new to Image Deconvolution and have some questions and it would be
great to get some answers/suggestions.
I was searching the ImageJ website for posts regarding Deconvolution plugins
in ImageJ that can match results from Huygens, AutoQuant etc. The last
thread was started by Christophe Leterrier and seems like there are no new
plugins because of limitations with the speed of Java. Is it still the case
or there are any new plugins being developed?

If you have any experience with the existing plugins, I would appreciate
suggestions/comments.
I tried Bob Dougherty's plugins which albeit slow, after writing macros, I
could manage it to run overnight and deconvolve about 70 stacks (256 x 256 x
50). Althought it seems pretty good I noticed that PSF generated with
different number of slices of PSF in Diffraction PSF 3D gives different
results with the Iterative Deconvolve 3D.  Is there any way to optimize
this? If I have a image stack with 50 z-slices, what should the ideal number
of slices I should have in the PSF?
Further, the more I have been reading, it seems that the best PSF is
generated from images of fluorescent beads that would take into account all
the inherent aberrations in the optics of the system being used (in this
case Zeiss
LSM510). Are there any plugins that can do this in ImageJ.

My attempts with Pierre Besson's (BIG) plugins haven't met much success so
far. Has anybody had any luck with this?

I would be glad to hear any suggestions/comments.

Thanks and wishes,
Hari

PS: My major application is to run a colocalization anylsis on the confocal
stacks I have.

Post-doc Fellow,
Oncology Center,
JHMI, Baltimore
MD 21231
USA