Re: Deconvolution, PSF, Dougherty's, Besson's plugins....

Posted by lechristophe on Mar 19, 2007; 9:41pm
URL: http://imagej.273.s1.nabble.com/Deconvolution-PSF-Dougherty-s-Besson-s-plugins-tp3700007p3700008.html

I'd be interested also to see an update to this topic !

Christophe Leterrier

Hari Iyer a écrit :

> Dear ImageJ users,
> I am very new to Image Deconvolution and have some questions and it
> would be
> great to get some answers/suggestions.
> I was searching the ImageJ website for posts regarding Deconvolution
> plugins
> in ImageJ that can match results from Huygens, AutoQuant etc. The last
> thread was started by Christophe Leterrier and seems like there are no new
> plugins because of limitations with the speed of Java. Is it still the case
> or there are any new plugins being developed?
>
> If you have any experience with the existing plugins, I would appreciate
> suggestions/comments.
> I tried Bob Dougherty's plugins which albeit slow, after writing macros, I
> could manage it to run overnight and deconvolve about 70 stacks (256 x
> 256 x
> 50). Althought it seems pretty good I noticed that PSF generated with
> different number of slices of PSF in Diffraction PSF 3D gives different
> results with the Iterative Deconvolve 3D.  Is there any way to optimize
> this? If I have a image stack with 50 z-slices, what should the ideal
> number
> of slices I should have in the PSF?
> Further, the more I have been reading, it seems that the best PSF is
> generated from images of fluorescent beads that would take into account all
> the inherent aberrations in the optics of the system being used (in this
> case Zeiss
> LSM510). Are there any plugins that can do this in ImageJ.
>
> My attempts with Pierre Besson's (BIG) plugins haven't met much success so
> far. Has anybody had any luck with this?
>
> I would be glad to hear any suggestions/comments.
>
> Thanks and wishes,
> Hari
>
> PS: My major application is to run a colocalization anylsis on the confocal
> stacks I have.
>
> Post-doc Fellow,
> Oncology Center,
> JHMI, Baltimore
> MD 21231
> USA
>